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Image Search Results
Journal: Frontiers in Veterinary Science
Article Title: Evaluation of stability and safety of equine mesenchymal stem cells derived from amniotic fluid for clinical application
doi: 10.3389/fvets.2024.1330009
Figure Lengend Snippet: Safety evaluation analysis of AF-MSCs. Karyotype of AF-MSCs at passage 5, 22, 42, 52 showing 31 pairs of autosome and 1 pair of sex chromosome, a normal karyotype of horse (A) . Mycoplasma contamination test by PCR (B) . When mycoplasma is contaminated, bands appear in 264 to 277 base pairs. A band of the internal control appears in the 170 base pair as a negative control. In AF-MSC, bands appeared only at the internal control position. This indicates that AF-MSC is mycoplasma negative. The band that appears <100 base pairs is primer dimer. Bar graph representation of cell viability as determined by MTT assay (C) . Cell viability of AF-MSCs in the “Live cell” and “Negative control” treated with 3% DMSO was represented as the percentage of absorbance at 570 nm. The statistically significant difference between the two groups, denoted by the double asterisks (** p < 0.01).
Article Snippet: Mycoplasma contamination was assessed using the
Techniques: Negative Control, MTT Assay